Assays - Minocycline

Determination of Minocycline in human plasma by high-performance liquid chromatography with UV detection after liquid-liquid extraction

Author: H.J. Mascher
Publisher: J. Chromatogr. A, 812 (1998), 339 - 342

A sensitive and specific high-performance liquid chromatographic method was developed and validaten for the determination of minocycline in human plasma. The method uses liquid-liquid extraction, reextraction and HPLC with UV detection. The assay shows linearity in the tested range of 28 - 3533 ng/ml with a limit of quantification of 30 ng/ml. The inter-day precision was found to be ± 3.84 to ± 6.57 % (RSD) in the range of 148 to 2743 ng/ml. The method was successfully applied to pharmacokinetic studies.