Assays - Indomethacin

Liquid-chromatographic determination of plasma Indomethacin in premature infants with patent ductus arteriosus

Author: A. Pollak, M. Weninger, H.R. Salzer, U. Salzermuhar, S. Levin, H.J. Mascher
Publisher: IRCS Medical science-biochemistry 14 (1986) 813-814

Indomethacin (I) and 8-methoxypsoralen (internal standard) were extracted from plasma with CHCl3 and separated by HPLC on a column of Microspher C-18 with a mobile phase of methanol - H3PO4 of pH 3.5 (3:1) and 320-nm detection. Response was linear from 0.7 to 4.0 mg/l and ≤75 µg/l of indomethacin could be detected. Recovery was 82% and inter-assay coefficient of variation were 6.8 and 3.9% for 150 and 385 µg/l of indomethacin, respectively.