Assays - Cimetidine

New rapid assay of Cimetidine in human plasma by reversed-phase high-performance liquid chromatography.

Author: V. Nitsche, H.J. Mascher
Publisher: J. Chromatogr. 273 (1983), 449-452

Cimetidine is a potent inhibitor of gastric acid secretion and is used worldwide with increasing frequency in the treatment of duodenal and stomach ulcers. Earlier dosage directions of 1000 mg of cimetidine per day have now been reduced to values of 400 mg twice daily. It is therefore important to note not only the clinically demonstrable effect, but also the therapeutically effective serum levels. So far, high-performance liquid chromatographic data have been published only for normal phase chromatography, three papers on reversed phase, and one paper using a weak polar phase. The aim of this present publication is the elaboration of a rapid assay for cimetidine, which provides not only an easy procedure and a short analysis time, but also a high recovery rate, making it the method of choice for the determination of cimetidine in biological material, where this is linked with pharmacokinetic examinations.