Assays - Benzbromarone

Detection of Benzbromarone in serum after combined therapy with Allopurinol

Author: V. Nitsche, H.J. Mascher
Publisher: Arzneim.-Forsch./Drug Res. 31 (1981), 510-512

A rapid method is described for benzbromarone assay in human serum. Protein precipitation is followed by extraction of the active substance. After centrifugation the clear organic layer is evaporated to dryness, redissolved in methanol and injected on a HPLC-column. Detection limits for this method of assay are 0.1 µg benzbromarone/ ml serum. The blood level for therapeutic concentrations lies between 1 and 2.5 µg/ml. Some pharmacokinetic data were presented. The half-life of benzbromarone is 2.6 h.